Patho-Genes.org: a website dedicated to gene sequences of potential bioterror bacteria and PCR primers used to amplify them
Article first published online: 10 JUN 2012
Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
Thematic Issue: Bioterrorism Research
Volume 5, Issue 5, pages 594–598, September 2012
How to Cite
Gardès, J., Bachar, D., Croce, O. and Christen, R. (2012), Patho-Genes.org: a website dedicated to gene sequences of potential bioterror bacteria and PCR primers used to amplify them. Microbial Biotechnology, 5: 594–598. doi: 10.1111/j.1751-7915.2012.00353.x
- Issue published online: 23 AUG 2012
- Article first published online: 10 JUN 2012
- Manuscript Accepted: 15 MAY 2012
- Manuscript Revised: 24 APR 2012
- Manuscript Received: 28 SEP 2011
- Direction Générale de l'Armement
- Ministère de la Défense Française
Pathogenic agents can be very hard to detect, and usually they do not cause illness for several hours or days. To improve the speed and the accuracy of detection tests and satisfy the needs of early diagnosis, molecular biology methods such as PCR are now used. However, selecting a proper target gene and designing good primers is often not easy. We present a dedicated website, http://patho-genes.org, where we provide every sequence, functional annotation, published primer and relevant article for every annotated gene of major pathogenic bacterial species listed as key agents to be used for a bioterrorism attack. Each published primer was analysed to determine its melting temperature, its specificity and its coverage (i.e. its sensitivity against every allele of its target gene). Data generated have been organized in the form of data sheet for each gene, which are available through multiple browser panels and query systems.