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  • Open Access

Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

Authors


  • Funding Information This work was supported by the Helmholtz Association (Helmholtz Grant VH-NG-716 to J.F.) and by the BMBF in frame of the SysMo2 programme.

For correspondence. E-mail j.frunzke@fz-juelich.de; Tel. (+49) 2461 61 5430; Fax (+49) 2461 61 2710.

Summary

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.

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