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Figure S1. Polymorphic residues of the 154-amino acid alignment of apyrase (First nucleotide: 166 in GenBank accession AY845193, Phlebotomus ariasi), with single alleles representing each Phlebotomus species except P. ariasi, which is represented by all amino acid alleles shared by at least two French or Spanish populations and those predominating in Morocco and Portugal.

Figure S2. Parsimony network (TCS v1.21) showing the genealogical relationships between the numbered nucleotide alleles of the apyrase of Phlebotomus ariasi (APYaN), indicating the absence of sibling species (nine-step 95% connection limit); reticulations, indicative of recurrent mutations or recombination; the presence in Morocco of alleles from only two parts of the network, one not shared with other regions; and the absence of allele 01 from Morocco and Portugal.

Figure S3. Association between genetic distances (FST/1−FST) based on apyrase nucleotide sequences and straight-line geographical distances for pairs of populations of Phlebotomus ariasi.

Table S1. Origins of sandflies, Phlebotomus ariasi populations and other Phlebotomus, characterized for apyrase.

Table S2. Quantitative sequence variation among the predominant alleles of the apyrases of Phlebotomus species.

Table S3. Hierarchical amova statistics for the apyrase of Phlebotomus ariasi to demonstrate the regional clustering of its populations.

FilenameFormatSizeDescription
EVA_226_sm_FigS1-S3.pdf309KSupporting info item
EVA_226_sm_TableS1.pdf371KSupporting info item
EVA_226_sm_TableS2.pdf529KSupporting info item
EVA_226_sm_TableS3.pdf81KSupporting info item

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