Table S1. Comparison of changes in differentiation observed between historical and contemporary periods based on different estimators.

Table S2. Temporal estimates of the effectivepopulation size (Ne) based on historical andcontemporary samples, assuming open populations and a generationlength (T) of 2 years.

Appendix S1. Details of laboratory methods used in processing archived tissue samples and methods of quality control.

Appendix S2. Details and results of coalescent simulations to examine loss of genetic diversity at constant population size.

eva233_sm_appendix1.doc34KSupporting info item
eva233_sm_appendix2.doc45KSupporting info item
eva233_sm_appendix3.doc31KSupporting info item
eva233_sm_appendix4.doc146KSupporting info item

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