Genomic regions in crop–wild hybrids of lettuce are affected differently in different environments: implications for crop breeding
Article first published online: 23 FEB 2012
© 2012 The Authors. Evolutionary Applications published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Volume 5, Issue 6, pages 629–640, September 2012
How to Cite
Hartman, Y., Hooftman, D. A. P., Uwimana, B., van de Wiel, C. C. M., Smulders, M. J. M., Visser, R. G. F. and van Tienderen, P. H. (2012), Genomic regions in crop–wild hybrids of lettuce are affected differently in different environments: implications for crop breeding. Evolutionary Applications, 5: 629–640. doi: 10.1111/j.1752-4571.2012.00240.x
- Issue published online: 6 SEP 2012
- Article first published online: 23 FEB 2012
- Received: 12 December 2011 Accepted: 21 December 2011
- crop–wild hybrids;
- Lactuca ;
- mitigation strategy;
- quantitative trait loci;
- transgenic plants
Many crops contain domestication genes that are generally considered to lower fitness of crop–wild hybrids in the wild environment. Transgenes placed in close linkage with such genes would be less likely to spread into a wild population. Therefore, for environmental risk assessment of GM crops, it is important to know whether genomic regions with such genes exist, and how they affect fitness. We performed quantitative trait loci (QTL) analyses on fitness(-related) traits in two different field environments employing recombinant inbred lines from a cross between cultivated Lactuca sativa and its wild relative Lactuca serriola. We identified a region on linkage group 5 where the crop allele consistently conferred a selective advantage (increasing fitness to 212% and 214%), whereas on linkage group 7, a region conferred a selective disadvantage (reducing fitness to 26% and 5%), mainly through delaying flowering. The probability for a putative transgene spreading would therefore depend strongly on the insertion location. Comparison of these field results with greenhouse data from a previous study using the same lines showed considerable differences in QTL patterns. This indicates that care should be taken when extrapolating experiments from the greenhouse, and that the impact of domestication genes has to be assessed under field conditions.