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Figure S1. Bayesian analysis of seven microsatellite loci showing the best clustering result (k = 2), as implemented with the Structure program. Specimens from the PAC region are in cluster one and specimens from the TMVB region are in cluster two.

Figure S2. Neighbor-joining tree of cox1 sequence divergences in S.  hellwegeri from the TMVB (TAP = Tapalpa, TEC = Tecalitlán and CAR = Careyes) and PAC regions (PAR = El Paraíso). Numbers in internal branches are bootstrap values. Scale bar represents the number of substitutions per site.

Table S1. amova of genetic distances within and between populations of S.  hellwegeri.

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