Historical specimens reveal past relationships and current conservation status of populations in a declining species: the regal fritillary butterfly
Version of Record online: 14 JUN 2012
© 2012 The Authors. Insect Conservation and Diversity © 2012 The Royal Entomological Society
Insect Conservation and Diversity
Volume 6, Issue 3, pages 234–242, May 2013
How to Cite
KEYGHOBADI, N., KOSCINSKI, D., WEINTRAUB, J. D. and FONSECA, D. M. (2013), Historical specimens reveal past relationships and current conservation status of populations in a declining species: the regal fritillary butterfly. Insect Conservation and Diversity, 6: 234–242. doi: 10.1111/j.1752-4598.2012.00208.x
- Issue online: 22 MAY 2013
- Version of Record online: 14 JUN 2012
- Accepted 1 April 2012 Editor: Koos Boomsma Associate editor: Thomas Schmitt
- Ancient DNA;
- conservation genetics;
- Speyeria idalia ;
Abstract. 1. The regal fritillary butterfly, Speyeria idalia Drury 1773, was once widespread across eastern North America, but has declined significantly and rapidly over the past half-century. Although more stable in the western portion of its range, only two populations survive east of the Great Lakes, one in eastern Pennsylvania and the other in Virginia.
2. Previous studies have found that the remnant Pennsylvania population is genetically differentiated from populations in the west, and have suggested the designation of separate eastern and western subspecies. However, the historical pattern of genetic variation from which the current distinctness of the Pennsylvania population has arisen was not known, nor was the relationship with the remnant Virginia population.
3. We amplified and sequenced two mitochondrial loci (COI/II and ND4) from preserved specimens to infer historical patterns of genetic variation in this species, and we used non-lethally obtained tissue samples to assess the relationship of the two eastern remnant populations.
4. We found very consistent patterns between the two loci. Both had a very shallow haplotype network with few mutations separating most haplotypes. At both loci, we observed distinct groups of haplotypes in the western and far eastern (i.e. New England) portions of the range; a region of transition was centred on Ohio, western Pennsylvania, and the Virginias, where both groups, and intermediate haplotypes, were represented.
5. Importantly, the extant Virginia population shared haplotypes with western populations of S. idalia and not with the extant Pennsylvania population. We discuss the implications of this result for the taxonomy and translocations/introductions of the species.