Authorship and contributorship: Lars-Georg Hersoug contributed to data collection, study design, analysed data, and wrote the paper; Charlotte Brasch-Andersen developed assays and supervised genotyping; Lise Lotte Nystrup Husemoen supervised data analyses; Torben Sigsgaard contributed data analyses; Allan Linneberg designed and planned the study, supervised data analyses. All authors contributed to the discussion of results, critically revising the paper, and have approved the final version of the paper.
The relationship of glutathione-S-transferases copy number variation and indoor air pollution to symptoms and markers of respiratory disease
Article first published online: 18 JUL 2011
© 2011 Blackwell Publishing Ltd
The Clinical Respiratory Journal
Volume 6, Issue 3, pages 175–185, July 2012
How to Cite
Hersoug, L.-G., Brasch-Andersen, C., Husemoen, L. L. N., Sigsgaard, T. and Linneberg, A. (2012), The relationship of glutathione-S-transferases copy number variation and indoor air pollution to symptoms and markers of respiratory disease. The Clinical Respiratory Journal, 6: 175–185. doi: 10.1111/j.1752-699X.2011.00258.x
Ethics: All participants gave written informed consent; the study protocol was approved by the local ethics committee of Copenhagen County and the danish Data Protection Agency.
Conflicts of interest: The authors have declared that they have no conflicts of interest.
- Issue published online: 14 JUN 2012
- Article first published online: 18 JUL 2011
- Accepted manuscript online: 8 JUN 2011 08:34AM EST
- Received: 30 May 2010; Revision requested: 16 March 2011; Accepted: 07 April 2011
- environmental tobacco smoke;
- glutathione S-transferases;
- particulate matter;
- respiratory symptoms;
Introduction: Exposure to particulate matter (PM) may induce inflammation and oxidative stress in the airways. Carriers of null polymorphisms of glutathione S-transferases (GSTs), which detoxify reactive oxygen species, may be particularly susceptible to the effects of PM.
Objectives: To investigate whether deletions of GSTM1 and GSTT1 modify the potential effects of exposure to indoor sources of PM on symptoms and objective markers of respiratory disease.
Methods: We conducted a population-based, cross-sectional study of 3471 persons aged 18–69 years. Information about exposure to indoor sources of PM and respiratory symptoms was obtained by a self-administered questionnaire. In addition, measurements of lung function (spirometry) and fractional exhaled nitric oxide were performed. Copy number variation of GSTM1 and GSTT1 was determined by polymerase chain reaction-based assays.
Results: We found that none of the symptoms and objective markers of respiratory disease were significantly associated with the GST null polymorphisms. An increasing number of positive alleles of the GSTM1 polymorphism tended to be associated lower prevalence of wheeze, cough, and high forced expiratory volume in 1 s (FEV1), but these trends were not statistically significant. Furthermore, we did not observe any statistically significant interactions between GST copy number variation and exposure to indoor sources of PM in relation to respiratory symptoms and markers.
Conclusions: In this adult population, GST copy number variations were not significantly associated with respiratory outcomes and did not modify the effects of self-reported exposure to indoor sources of PM on respiratory outcomes.
Please cite this paper as: Hersoug L-G, Brasch-Andersen C, Husemoen LLN, Sigsgaard T and Linneberg A. The relationship of glutathione-S-transferases copy number variation and indoor air pollution to symptoms and markers of respiratory disease. Clin Respir J 2012; 6: 175–185.