Development of cytochrome b primers for mitotyping of barbels (Barbus spp.)

Authors

  • H. F. GANTE,

    1. School of Life Sciences, LSC 274, Arizona State University, Tempe, AZ 85287-4601, USA,
    2. Centro de Biologia Ambiental and Museu Bocage — Museu Nacional de História Natural, Rua da Escola Politécnica 58, 1269-102 Lisboa, Portugal
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  • M. J. ALVES,

    1. Centro de Biologia Ambiental and Museu Bocage — Museu Nacional de História Natural, Rua da Escola Politécnica 58, 1269-102 Lisboa, Portugal
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  • T. E. DOWLING

    1. School of Life Sciences, LSC 274, Arizona State University, Tempe, AZ 85287-4601, USA,
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Hugo F. Gante, Fax: +351 213969784; E-mail: hugo.gante@asu.edu

Abstract

Primers were developed that allow for rapid, reliable and inexpensive screening of cytochrome b by analysis of single-stranded conformational polymorphisms. Twenty different haplotypes were identified from six species of Iberian Barbus. These primers proved useful for population and species level studies, and could also be valuable in population genetic and phylogenetic studies of other cyprinin fishes.

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