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Eleven polymorphic microsatellites isolated from red swamp crayfish, Procambarus clarkii

Authors

  • ZE YUAN ZHU,

    1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore
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  • GEN HUA YUE

    1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore
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Dr Gen Hua Yue, Fax: 65 68727007; E-mail: genhua@tll.org.sg

Abstract

Eleven microsatellites were isolated from a genomic DNA library enriched for CA- and GA-repeats, and were characterized in 48 individuals of red swamp crayfish Procambarus clarkii. All 11 microsatellites were polymorphic with an average of allele number of 6.6 per locus. Genotypic distributions of these 11 markers were found to be in conformance with the expectations of Hardy–Weinberg equilibrium. All 11 markers were unlinked. These markers are being used to study the invasion routine, genetic diversity and population structure of the species P. clarkii.

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