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Isolation and characterization of microsatellite markers from the marine isopods Serolis paradoxa and Septemserolis septemcarinata (Crustacea: Peracarida)

Authors

  • FLORIAN LEESE,

    1. Alfred Wegener Institute for Polar and Marine Research, Marine Animal Ecology PO Box 12 0161, D-27515 Bremerhaven, Germany,
    2. Department of Animal Ecology, Evolution and Biodiversity, Ruhr University of Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany,
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  • ANNA KOP,

    1. Alfred Wegener Institute for Polar and Marine Research, Marine Animal Ecology PO Box 12 0161, D-27515 Bremerhaven, Germany,
    2. York University, 4700 Keele St., Toronto, Canada M3J 1P3
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  • SHOBHIT AGRAWAL,

    1. Alfred Wegener Institute for Polar and Marine Research, Marine Animal Ecology PO Box 12 0161, D-27515 Bremerhaven, Germany,
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  • CHRISTOPH HELD

    1. Alfred Wegener Institute for Polar and Marine Research, Marine Animal Ecology PO Box 12 0161, D-27515 Bremerhaven, Germany,
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Florian Leese, Fax: +49(0)471.4831– 1918; E-mail: florian.leese@awi.de

Abstract

This study reports the successful isolation of highly informative microsatellite marker sets for two marine serolid isopod species. For Serolis paradoxa (Fabricius, 1775), 13, and for Septemserolis septemcarinata (Miers, 1875), eight polymorphic microsatellite markers were isolated using the reporter genome enrichment protocol. The number of alleles per locus (NA) and the observed heterozygosity (HO) encompass a wide range of variation within S. paradoxa (NA 3–31, HO 6–89%) and S. septemcarinata (NA 2–18, HO 9–94%). The suitability of the newly isolated markers for population genetic studies is evaluated.

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