Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

Authors

  • HANNAH MCPHERSON,

    1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia,
    2. Ecosystem Management, University of New England, Armidale, NSW 2351, Australia
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  • CAROLYN PORTER,

    1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia,
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  • PAUL D. RYMER,

    1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia,
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  • DARREN M. CRAYN,

    1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia,
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  • MAURIZIO ROSSETTO

    1. National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia,
    2. Ecosystem Management, University of New England, Armidale, NSW 2351, Australia
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Hannah McPherson, Fax: +61-2-9251 7231; E-mail: hannah.mcpherson@rbgsyd.nsw.gov.au

Abstract

We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

Ancillary