Isolation and characterization of polymorphic microsatellite and COI loci from the whelk Kelletia kelletii

Authors


Crow White, Fax: 805-893-7612; E-mail: crowsfeather@gmail.com

Abstract

There is considerable interest in the genetic structure of Kelletia kelletii because of its economic, ecological and scientific importance. To that end, we developed species-specific primers which amplify mitochondrial cytochrome c oxidase subunit I as well as 13 hypervariable nuclear microsatellite loci. Using dye-labelled primers, the microsatellite loci can be co-amplified in two multiplex polymerase chain reactions and scored simultaneously on an automated sequencer.

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