Cross-amplification and characterization of 13 tetranucleotide microsatellites in multiple species of Neotropical canids

Authors

  • MANOEL L. DA FONTOURA-RODRIGUES,

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
    2. Departamento de Genética, Instituto de Biociências, UFRGS, Av. Bento Gonçalves 9500, Prédio 43323, Porto Alegre, RS 91501-970, Brazil,
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  • CARLOS ANDRÉ V. LIMA-ROSA,

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
    2. Departamento de Genética, Instituto de Biociências, UFRGS, Av. Bento Gonçalves 9500, Prédio 43323, Porto Alegre, RS 91501-970, Brazil,
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  • LIGIA TCHAICKA,

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
    2. Departamento de Genética, Instituto de Biociências, UFRGS, Av. Bento Gonçalves 9500, Prédio 43323, Porto Alegre, RS 91501-970, Brazil,
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  • FERNANDA P. VALDEZ,

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
    2. Departamento de Genética, Instituto de Biociências, UFRGS, Av. Bento Gonçalves 9500, Prédio 43323, Porto Alegre, RS 91501-970, Brazil,
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  • FLÁVIO H. G. RODRIGUES,

    1. Instituto Pró-Carnívoros, Brazil,
    2. Departamento de Biologia Geral, ICB, UFMG, Belo Horizonte, MG 31270-910, Brazil,
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  • ROGÉRIO C. DE PAULA,

    1. Instituto Pró-Carnívoros, Brazil,
    2. Centro Nacional de Pesquisas para Conservação dos Predadores Naturais — CENAP/IBAMA, Brazil,
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  • MICHAEL P. GOUGH,

    1. Laboratory of Genomic Diversity, NCI-Frederick, Frederick, MD 21702-1201, USA
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  • WARREN E. JOHNSON,

    1. Laboratory of Genomic Diversity, NCI-Frederick, Frederick, MD 21702-1201, USA
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  • SANDRO L. BONATTO,

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
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  • EDUARDO EIZIRIK

    1. Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil,
    2. Instituto Pró-Carnívoros, Brazil,
    3. Laboratory of Genomic Diversity, NCI-Frederick, Frederick, MD 21702-1201, USA
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Eduardo Eizirik, Fax: 55-51-3320-3612; E-mail: eduardo.eizirik@pucrs.br

Abstract

We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 × 10−9. This set of loci has a great potential for application in evolutionary, ecological and conservation studies.

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