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New polymorphic microsatellite markers for the limpet Patella rustica and cross-priming testing in four Patella species

Authors

  • M. PÉREZ,

    1. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal,
    2. Department of Biochemistry, Genetics and Immunology, Faculty of Marine Sciences, University of Vigo, 36310 Vigo, Spain,
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  • A. LLAVONA,

    1. Department of Biochemistry, Genetics and Immunology, Faculty of Marine Sciences, University of Vigo, 36310 Vigo, Spain,
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  • P. PRESA,

    1. Department of Biochemistry, Genetics and Immunology, Faculty of Marine Sciences, University of Vigo, 36310 Vigo, Spain,
    2. ECIMAT. Estación de Ciencias Marinas de Toralla, Universidad de Vigo, 36331 Vigo, Spain,
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  • P. RIBEIRO,

    1. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal,
    2. University of Southampton, Southampton S09 5NH, UK
    3. Marine Biological Association, Plymouth PL1 2PB, UK
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  • S. J. HAWKINS,

    1. University of Southampton, Southampton S09 5NH, UK
    2. Marine Biological Association, Plymouth PL1 2PB, UK
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  • A. M. SANTOS,

    1. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal,
    2. Departamento de Zoologia-Antropologia, Faculdade de Ciências da Universidade do Porto, 4099-002 Porto, Portugal,
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  • P. ALEXANDRINO

    1. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal,
    2. Departamento de Zoologia-Antropologia, Faculdade de Ciências da Universidade do Porto, 4099-002 Porto, Portugal,
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Montse Pérez, Fax: +34 986 812567; E-mail: mon@uvigo.es

Abstract

Eighteen microsatellite markers have been characterized from the Patella rustica genome. An average of 10 alleles per locus and an expected heterozygosity ranging from 0.054 to 0.937 were observed in a sample of 32 wild individuals from Viana do Castelo, Portugal. Distinct cross-priming amplification rates were recovered on four additional Patella species. Three monomorphic P. rustica loci were polymorphic in other Patella species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine population studies in the genus Patella.

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