Characterization of eight single nucleotide polymorphism markers in Aedes aegypti
Article first published online: 15 SEP 2008
DOI: 10.1111/j.1755-0998.2008.02282.x
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Additional Information
How to Cite
PADUAN, K. and RIBOLLA, P. (2009), Characterization of eight single nucleotide polymorphism markers in Aedes aegypti. Molecular Ecology Resources, 9: 114–116. doi: 10.1111/j.1755-0998.2008.02282.x
Publication History
- Issue published online: 22 JAN 2009
- Article first published online: 15 SEP 2008
- Received 31 January 2008; revision accepted 7 April 2008
Keywords:
- Aedes aegypti;
- genetic markers;
- genotyping;
- minisequencing;
- PCR;
- single nucleotide polymorphism
Abstract
Sequencing of part of seven genes from Aedes aegypti collected in 16 Brazilian cities revealed the existence of 53 single nucleotide polymorphisms (SNPs), representing one SNP every 52 base pairs. From these 53 SNPs, we selected eight that are independent and highly polymorphic. We describe the use of these markers for differentiation of Brazilian populations of A. aegypti. These are the first SNPs developed for delineating population structure in A. aegypti, and will be a useful complement to epidemiological studies.

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