Characterization of eight single nucleotide polymorphism markers in Aedes aegypti

Authors

  • KS PADUAN,

    1. Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Caixa Postal 510, 18618-000, Botucatu, São Paulo, Brazil
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  • PEM RIBOLLA

    1. Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Caixa Postal 510, 18618-000, Botucatu, São Paulo, Brazil
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Karina dos Santos Paduan, Fax: +55 14 38153744; E-mail: kapaduan@yahoo.com.br

Abstract

Sequencing of part of seven genes from Aedes aegypti collected in 16 Brazilian cities revealed the existence of 53 single nucleotide polymorphisms (SNPs), representing one SNP every 52 base pairs. From these 53 SNPs, we selected eight that are independent and highly polymorphic. We describe the use of these markers for differentiation of Brazilian populations of A. aegypti. These are the first SNPs developed for delineating population structure in A. aegypti, and will be a useful complement to epidemiological studies.

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