Get access

Isolation and characterization of microsatellite loci in tropical forage Stylosanthes capitata Vogel

Authors

  • M. O. SANTOS,

    1. Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Universidade Estadual de Campinas, CP 6010, Barão Geraldo, Campinas, SP, Brazil, CEP 13083-970,
    Search for more papers by this author
  • R. P. SASSAKI,

    1. Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Universidade Estadual de Campinas, CP 6010, Barão Geraldo, Campinas, SP, Brazil, CEP 13083-970,
    Search for more papers by this author
  • L. CHIARI,

    1. Empresa Brasileira de Pesquisa Agropecuária (Embrapa) — Gado de Corte, BR 262, Km 4, Campo Grande, MS, Brazil, CEP 79002-970
    Search for more papers by this author
  • R. M. S. RESENDE,

    1. Empresa Brasileira de Pesquisa Agropecuária (Embrapa) — Gado de Corte, BR 262, Km 4, Campo Grande, MS, Brazil, CEP 79002-970
    Search for more papers by this author
  • A. P. DE SOUZA

    1. Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Universidade Estadual de Campinas, CP 6010, Barão Geraldo, Campinas, SP, Brazil, CEP 13083-970,
    Search for more papers by this author

Anete Pereira de Souza, Fax: 55-19-3521-1089; E-mail: anete@unicamp.br

Abstract

Stylosanthes capitata is an important tropical pasture legume. Knowledge of genetic diversity and structure of S. capitata populations is of great importance for the conservation and germplasm management of this species. Thus, eight microsatellite markers were developed from an S. capitata-enriched library. They were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The observed and expected heterozygosities ranged from 0.16 to 0.85 and from 0.40 to 0.85, respectively. These microsatellites are the first set of molecular markers from this species and will contribute towards studies of genetic diversity, conservation and breeding of S. capitata.

Get access to the full text of this article

Ancillary