Development and characterization of 14 polymorphic microsatellite loci in the raccoon tick (Ixodes texanus)

Authors


Guha Dharmarajan, Fax: (765) 496-2422; E-mail: gdharmar@purdue.edu

Abstract

Fourteen polymorphic microsatellite loci were developed for the raccoon tick (Ixodes texanus). Three multiplexed panels comprising the loci were developed and 50 ticks collected from two infected raccoons (Procyon lotor) were genotyped. The number of alleles per locus ranged from five to 22, and single locus heterozygosities ranged from 0.46 to 0.94. Future research will further our understanding of I. texanus biology and help in elucidating the effects of life-history variation on parasite population genetic structure, using I. texanus as a model organism.

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