Isolation and characterization of microsatellites in the woody shrub, Banksia sphaerocarpa var. caesia (Proteaceae)

  1. H. M. NISTELBERGER,
  2. T. M. LLORENS,
  3. M. BYRNE

Article first published online: 10 OCT 2008

DOI: 10.1111/j.1755-0998.2008.02323.x

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 9, Issue 1, pages 148–149, January 2009

Additional Information

How to Cite

NISTELBERGER, H. M., LLORENS, T. M. and BYRNE, M. (2009), Isolation and characterization of microsatellites in the woody shrub, Banksia sphaerocarpa var. caesia (Proteaceae). Molecular Ecology Resources, 9: 148–149. doi: 10.1111/j.1755-0998.2008.02323.x

Author Information

  1. Science Division, Department of Environment and Conservation, Locked Bag 104, Bentley Delivery Centre, WA 6983, Australia

* T. M. Llorens, Fax: +61 8 9334 0327; E-mail: tanya.llorens@dec.wa.gov.au

Publication History

  1. Issue published online: 22 JAN 2009
  2. Article first published online: 10 OCT 2008
  3. Received 8 May 2008; revision accepted 1 July 2008

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Keywords:

  • Banksia sphaerocarpa;
  • gene flow;
  • microsatellites;
  • primers

Abstract

Microsatellite markers were developed for the Australian bird-pollinated woody shrub Banksia sphaerocarpa var. caesia to study gene flow among populations in a highly fragmented landscape. Eight loci were developed, and in a sample of 40 individuals from one population, the number of alleles per locus ranged from five to 21 and observed heterozygosities ranged from 0.385 to 0.914. All eight loci showed independent inheritance. Analysis of open-pollinated progeny arrays confirmed Mendelian inheritance at seven loci, while null alleles were suspected at the remaining locus.

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