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Non-invasive genetic identification of small mammal species using real-time polymerase chain reaction

Authors


Siobhan Moran, Fax: +35351 302679; E-mail: smoran@wit.ie

Abstract

DNA identification of non-invasive samples is a potentially useful tool for monitoring small mammal species. Here we describe a novel method for identifying five small mammal species: wood mouse, bank vole, common shrew, pygmy shrew and water shrew. Species-specific real-time polymerase chain reaction primers were designed to amplify fragments of the mitochondrial cytochrome b gene from hair and scat samples. We also amplified nuclear DNA from scats, demonstrating their potential as a source of DNA for population genetic studies.

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