Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F1 hybrids

  1. R HOBZA1,2,
  2. A WIDMER2

Article first published online: 29 OCT 2008

DOI: 10.1111/j.1755-0998.2008.02344.x

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 8, Issue 6, pages 1274–1276, November 2008

Additional Information

How to Cite

HOBZA, R. and WIDMER, A. (2008), Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F1 hybrids. Molecular Ecology Resources, 8: 1274–1276. doi: 10.1111/j.1755-0998.2008.02344.x

Author Information

  1. 1

    Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic,

  2. 2

    Plant Ecological Genetics, Institute of Integrative Biology, ETH Zurich, Universitaetstr. 16, 8092 Zurich, Switzerland

* Roman Hobza, Fax: + 420-541-240-500; E-mail: hobza@ibp.cz

Publication History

  1. Issue published online: 29 OCT 2008
  2. Article first published online: 29 OCT 2008
  3. Received 5 June 2008; revision accepted 18 July 2008

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Keywords:

  • hybrid;
  • paternity analysis;
  • sex chromosome;
  • sexing;
  • white campion

Abstract

Two polymerase chain reaction-based assays have been developed that work in combination with an efficient DNA extraction protocol to rapidly and reliably determine sex in the dioecious plant species Silene latifolia and S. dioica. In addition, one of the assays allows assessing paternity in the F1 generation of intra- and interspecific matings involving the two species.

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