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PERMANENT GENETIC RESOURCES NOTE
Isolation and characterization of 23 microsatellite loci in the yellow tang, Zebrasoma flavescens (Pisces: Acanthuridae)
Article first published online: 31 JAN 2009
DOI: 10.1111/j.1755-0998.2008.02354.x
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
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How to Cite
CHRISTIE, M. R. and EBLE, J. A. (2009), Isolation and characterization of 23 microsatellite loci in the yellow tang, Zebrasoma flavescens (Pisces: Acanthuridae). Molecular Ecology Resources, 9: 544–546. doi: 10.1111/j.1755-0998.2008.02354.x
Publication History
- Issue published online: 12 FEB 2009
- Article first published online: 31 JAN 2009
- Received 21 July 2008; revision accepted 1 August 2008
- Abstract
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Keywords:
- connectivity;
- coral-reef fish;
- Hawaii;
- Indo-Pacific;
- pelagic larval dispersal;
- surgeonfish
Abstract
Twenty-three microsatellites were isolated from the yellow tang (Zebrasoma flavescens), an ecologically and commercially important reef fish. Genetic diversity was assessed in 90 adults collected from Honokohau, Hawaii. The number of alleles per locus varied from four to 29 (mean = 13.8) and observed and expected heterozygosities ranged from 0.15 to 0.94 (mean = 0.70) and from 0.29 to 0.93 (mean = 0.81), respectively. Eight loci exhibited significant departure from Hardy–Weinberg equilibrium due to the presence of null alleles. Exact tests showed no evidence of genotypic disequilibrium between loci. Overall, loci were well resolved, easy to score and highly polymorphic.