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Development and characterization of eight polymorphic microsatellite markers for Daphnia atkinsoni (Crustacea: Ctenodaphnia)

Authors

  • R. ORTELLS,

    1. Laboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Charles Deberiotstraat, 32, B-3000 Leuven, Belgium,
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  • J. K. J. VAN HOUDT,

    1. Joint Experimental Molecular Unit, Royal Museum for Central Africa, Leuvensesteenweg 13, 3080 Tervuren, Belgium
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  • S. GELDOF,

    1. Laboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Charles Deberiotstraat, 32, B-3000 Leuven, Belgium,
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  • L. DE MEESTER,

    1. Laboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Charles Deberiotstraat, 32, B-3000 Leuven, Belgium,
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  • J. MERGEAY

    1. Laboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Charles Deberiotstraat, 32, B-3000 Leuven, Belgium,
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Joachim Mergeay, Fax: +32 16 32 4575; E-mail: joachim.mergeay@bio.kuleuven.be

Abstract

A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former) to high (in the latter) levels of polymorphism with two to 11 alleles per locus and an observed heterozygosity ranging from 0 to 0.87. Some of these microsatellite markers were successfully amplified in three other Daphnia species (D. magna n = 4, D. similis n = 6; D. obtusa n = 6).

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