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Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis

Authors

  • MATTHIEU LERAY,

    1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA,
    2. UMR 5244, CNRS-EPHE-UPVD, Centre de Biologie et d’Ecologie Tropicale et Méditerranéenne, Université de Perpignan, 66860 Perpignan Cedex, France,
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  • RICARDO BELDADE,

    1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA,
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  • SALLY J. HOLBROOK,

    1. Coastal Research Center, Marine Science Institute, and Department of Ecology, Evolution, and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA
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  • RUSSELL J. SCHMITT,

    1. Coastal Research Center, Marine Science Institute, and Department of Ecology, Evolution, and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA
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  • SERGE PLANES,

    1. UMR 5244, CNRS-EPHE-UPVD, Centre de Biologie et d’Ecologie Tropicale et Méditerranéenne, Université de Perpignan, 66860 Perpignan Cedex, France,
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  • GIACOMO BERNARDI

    1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA,
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Giacomo Bernardi, Fax: +1 831 459 33 83; E-mail: bernardi@biology.ucsc.edu

Abstract

A set of 13 simple sequence repeat markers was developed from D. trimaculatus genomic DNA, tested for D. auripinnis and characterized using 40 individuals per species. All the loci were polymorphic with a number of alleles ranging from three to 30. Observed heterozygosities varied from 0.23 to 0.89 for D. trimaculatus and from 0.11 to 0.85 for D. auripinnis. Early results show that these will be powerful markers for the study of ecological and evolutionary mechanism in this coral reef fish species complex.

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