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Isolation, characterization and cross-amplification of polymorphic microsatellite loci in the threatened endemic Mammillaria crucigera (Cactaceae)

Authors

  • SOFIA SOLÓRZANO,

    1. Unidad de Biología, Tecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. Barrios 1. Los Reyes Iztacala, Tlalnepantla, 54090 Estado de México, México,
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  • AUREA C. CORTÉS-PALOMEC,

    1. CIEco, Universidad Nacional Autónoma de México, Antigua carretera a Pátzcuaro, 8701 San José la Huerta, Morelia, 58190 Michoacán, México
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  • ARTURO IBARRA,

    1. Unidad de Biología, Tecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. Barrios 1. Los Reyes Iztacala, Tlalnepantla, 54090 Estado de México, México,
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  • PATRICIA DÁVILA,

    1. Unidad de Biología, Tecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. Barrios 1. Los Reyes Iztacala, Tlalnepantla, 54090 Estado de México, México,
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  • KEN OYAMA

    1. CIEco, Universidad Nacional Autónoma de México, Antigua carretera a Pátzcuaro, 8701 San José la Huerta, Morelia, 58190 Michoacán, México
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Sofia Solórzano, Fax: (+52) 55 56 23 12 25; E-mail: solorzanols@campus.iztacala.unam.mx

Abstract

The cactus Mammillaria crucigera is a threatened species endemic to central Mexico. As a means of assessing population genetic status of these species, eight microsatellite markers were developed. These primers were tested in 40 individual from two wild populations. The results showed that these primers will be useful to describe population structure and aid to the conservation of species. The eight primers were tested in other Mammillaria species and most of them showed successful amplification.

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