Characterization of 31 EST-derived microsatellite markers for the pearl oyster Pinctada martensii (Dunker)

Authors

  • YAOHUA SHI,

    1. State Key Biotechnology Laboratory of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, 571101, China,
    2. Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, Haikou 570228, China,
    3. Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Avenue, Port Norris, NJ 08349, USA
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  • YAN WANG,

    1. Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, Haikou 570228, China,
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  • KUI HONG,

    1. State Key Biotechnology Laboratory of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, 571101, China,
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  • ZHANHUI HOU,

    1. Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, Haikou 570228, China,
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  • AIMIN WANG,

    1. Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, Haikou 570228, China,
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  • XIMING GUO

    1. Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Avenue, Port Norris, NJ 08349, USA
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  • Proof-pages should be sent to X. Guo, 6959 Miller Avenue, Port Norris, NJ 08349, USA

A. Wang, E-mail: aimwang@163.com and X. Guo, E-mail: xguo@hsrl.rutgers.edu

Abstract

We developed and characterized 31 microsatellite markers from expressed sequence tags of Pinctada martensii (Dunker). The number of alleles per locus ranged from 4 to 18 as determined in 44 individuals from a wild population. The expected heterozygosity ranged from 0.4121 to 0.9436, while the observed heterozygosity ranged from 0.4054 to 0.7273. Most of the loci are in Hardy–Weinberg equilibrium. These markers should be useful for population genetics studies, parentage and genome mapping in this species.

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