Isolation and characterization of nine microsatellite loci for the tropical understory tree Miconia affinis Wurdack (Melastomataceae)

Authors

  • SHALENE JHA,

    1. Department of Ecology and Evolutionary Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109, USA,
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  • CHRISTOPHER W. DICK

    1. Department of Ecology and Evolutionary Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109, USA,
    2. Smithsonian Tropical Research Institute, PO Box 0843-03092, Balboa, Ancón, Republic of Panamá
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Shalene Jha, Fax: (734) 763-0544; E-mail: sjha@umich.edu

Abstract

We isolated nine microsatellite loci from the Neotropical understorey tree Miconia affinis (Melastomataceae) and optimized them for research on gene flow and genetic structure. Loci screened in 43 individuals from a 2.26-km2 region were shown to be unlinked and polymorphic, with 5 to 14 alleles per locus and observed heterozygosity ranging from 0.394 to 0.810.

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