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Keywords:

  • genetic diversity;
  • Miconia;
  • microsatellites;
  • parentage;
  • shade coffee

Abstract

We isolated nine microsatellite loci from the Neotropical understorey tree Miconia affinis (Melastomataceae) and optimized them for research on gene flow and genetic structure. Loci screened in 43 individuals from a 2.26-km2 region were shown to be unlinked and polymorphic, with 5 to 14 alleles per locus and observed heterozygosity ranging from 0.394 to 0.810.