Microsatellite loci characterized in three African crane species (Gruidae, Aves)

Authors

  • KATE MEARES,

    1. Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK,
    2. School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg 3209, KwaZulu-Natal, South Africa,
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  • DEBORAH A. DAWSON,

    1. Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK,
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  • GAVIN J. HORSBURGH,

    1. Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK,
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  • TRAVIS C. GLENN,

    1. Department of Environmental Health Science, University of Georgia, Athens, GA 30602-2102, USA,
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  • KENNETH L. JONES,

    1. Department of Environmental Health Science, University of Georgia, Athens, GA 30602-2102, USA,
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  • MICHAEL J. BRAUN,

    1. Department of Vertebrate Zoology, National Museum of Natural History, Smithsonian Institution, 4210 Silver Hill Road, Suitland, MD 20746, USA
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  • MIKE R. PERRIN,

    1. School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg 3209, KwaZulu-Natal, South Africa,
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  • TIAWANNA D. TAYLOR

    1. School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg 3209, KwaZulu-Natal, South Africa,
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Deborah Dawson, Fax: +44 (0)114 222 0002; E-mail: D.A.Dawson@Sheffield.ac.uk

Abstract

Forty-three microsatellite loci originally isolated in Grus americana and G. japonensis were tested for polymorphism in the blue crane (G. paradisea). Amplified products were sequenced in the blue crane to aid in the design of blue crane-specific primers. When characterized in 20 unrelated blue crane individuals from South Africa, 14 loci were polymorphic, with each locus displaying between 2 and 7 alleles. Eight polymorphic loci were characterized in the grey-crowned crane (Balearica regulorum) and ten in the wattled crane (G. carunculatus).

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