Isolation and characterization of 11 polymorphic trinucleotide microsatellite markers in the stonefly Arcynopteryx compacta (Plecoptera: Perlodidae)

Authors

  • KATHRIN THEISSINGER,

    1. Department of Limnology and Conservation, Research Institute Senckenberg, Germany
    2. Department of Molecular Ecology, Institute of Zoology, Johannes Gutenberg University, Mainz, Germany
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  • KEVIN A. FELDHEIM,

    1. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, Chicago, USA
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  • ALFRED SEITZ,

    1. Department of Molecular Ecology, Institute of Zoology, Johannes Gutenberg University, Mainz, Germany
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  • STEFFEN U. PAULS

    1. Department of Limnology and Conservation, Research Institute Senckenberg, Germany
    2. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, Chicago, USA
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Steffen U. Pauls, Department of Entomology, 219 Hodson Hall, University of Minnesota, 1980 Folwell Avenue, St Paul, MN 55108, USA. E-mail: pauls497@umn.edu

Abstract

We describe the isolation of 11 polymorphic trinucleotide microsatellite loci from the stonefly Arcynopteryx compacta. Loci were highly variable with 3 to 14 alleles (mean = 6.45). Observed heterozygosity ranged from 0 to 0.867. Seven loci showed significant deviation from Hardy–Weinberg equilibrium across both populations. There was no evidence for null alleles, and thus, Hardy–Weinberg departures could have resulted from genetic structure between populations or subpopulations. No linkage between loci was found. The 11 loci should prove highly informative for population genetic studies.

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