Development of polymorphic microsatellite markers for the human botfly, Dermatobia hominis (Diptera: Oestridae)

Authors

  • BÁRBARA DOMINGUES BITARELLO,

    1. Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética Universidade Estadual de Campinas – UNICAMP, CP 6010, 13083-875, Campínas, SP, Brazil
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  • TATIANA TEIXEIRA TORRES,

    1. Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética Universidade Estadual de Campinas – UNICAMP, CP 6010, 13083-875, Campínas, SP, Brazil
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  • MARIANA LÚCIO LYRA,

    1. Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética Universidade Estadual de Campinas – UNICAMP, CP 6010, 13083-875, Campínas, SP, Brazil
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  • ANA MARIA LIMA DE AZEREDO-ESPIN

    1. Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética Universidade Estadual de Campinas – UNICAMP, CP 6010, 13083-875, Campínas, SP, Brazil
    2. Departamento de Genética e Evolução, Instituto de Biologia, UNICAMP, CP 6109, 13083-970, Campinas, SP, Brazil
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B.D. Bitarello, Fax: +55-19-3521-1089; E-mail: bdbitarello@yahoo.com.br

Abstract

In this report, we describe the development of 17 polymorphic microsatellite markers for the human botfly, Dermatobia hominis, an obligatory parasite of mammals of great veterinary importance in Latin America. The number of alleles ranged from 5 to 21 per locus, with a mean of 12.2 alleles per locus. The expected heterozygosity ranged from 0.2571 to 0.9206 and from 0.2984 to 0.9291 in two populations from Brazil. These markers should provide a high resolution tool for assessment of the fine-scale genetic structure of natural populations of the human botfly.

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