Transferability and characterization of nine microsatellite markers for the tropical tree species Tabebuia roseo-alba

Authors

  • JULIANA MASSIMINO FERES,

    1. Plant Genetic Laboratory
    2. Graduate Program in Genetics, Department of Genetics, FMRP
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  • MARCELO L. L. MARTINEZ,

    1. Plant Genetic Laboratory
    2. Graduate Program in Comparative Biology, Department of Biology, FFCLRP, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-900, Ribeirao Preto, SP, Brazil
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  • CARLOS A. MARTINEZ,

    1. Graduate Program in Comparative Biology, Department of Biology, FFCLRP, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-900, Ribeirao Preto, SP, Brazil
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  • MOACYR A. MESTRINER,

    1. Plant Genetic Laboratory
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  • ANA LILIA ALZATE-MARIN

    1. Plant Genetic Laboratory
    2. Graduate Program in Genetics, Department of Genetics, FMRP
    3. Graduate Program in Comparative Biology, Department of Biology, FFCLRP, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-900, Ribeirao Preto, SP, Brazil
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  • (Juliana Massimino Feres, Marcelo L. L. Martinez) These two authors contributed equally to this work.

Ana Lilia Alzate-Marin, Fax: +55 16 36330069; E-mail: anaalzate@rge.fmrp.usp.br

Abstract

Microsatellite loci that were previously developed in the tropical tree Tabebuia aurea were used for the genetic analysis of Tabebuia roseo-alba populations. Nine of 10 simple sequence repeat markers were amplified, and the polymorphism was assessed in 58 individuals sampled from two stands in southeastern Brazil. All loci were polymorphic with Mendelian inheritance. The allele numbers were high, ranging from 5 to 13 in population I and 3 to 7 in population II, with means of 8.9 and 5.5, respectively. We conclude that these markers can be efficiently used for parentage and gene-flow studies.

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