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Isolation and characterization of microsatellite loci for storm-petrels

Authors

  • ZHENGXIN SUN,

    1. Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
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  • ELENA GÓMEZ-DÍAZ,

    1. Departament Biologia Animal, Universitat de Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain
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    • Present address: Génétique et Evolution des Maladies Infectieuses (GEMI) UMR, CNRS/IRD, 2724 Centre IRD Montpellier, 911 Av Agropolis-BP 64501, 34394 Montpellier Cedex 5, France.

  • ANNA BAILIE,

    1. Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
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    • §

      Present address: Department of Plant Science, McGill University, Macdonald Campus, 21,111 Lakeshore Road, Sainte-Anne-de-Bellevue, QC, Canada H9X 3V9.

  • VICKI FRIESEN

    1. Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
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  • doi: 10.1111/j.1755-0998.2008.02487.x

Vicki Friesen, Fax: 1-613-533-6617; E-mail: vlf@queensu.ca

Abstract

Primers were developed for 10 microsatellite loci for two species of Oceanodroma storm-petrels. Variability was tested in 27 O. castro and 22 O. monteiroi from the Azores, and 24 O. leucorhoa from Norway. At least six loci amplified reliably and were polymorphic in each species. The number of alleles per locus averaged 4.6, and observed heterozygosities averaged 0.41. Most primers also yielded polymerase chain reaction products in O. tethys, O. hornbyi and Pterodroma phaeopygia. These loci are being used to assay population genetic structure in storm-petrels.

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