Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India

Authors

  • B. T. RAMESHA,

    Corresponding author
    1. Department of Crop Physiology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    2. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
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  • R. SRIRAMA,

    1. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    2. Ashoka Trust for Research in Ecology and the Environment, 5A Main, Hebbal, Bangalore 560 024, India,
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  • G. RAVIKANTH,

    1. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    2. Ashoka Trust for Research in Ecology and the Environment, 5A Main, Hebbal, Bangalore 560 024, India,
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  • K. V. RAVISHANKAR,

    1. Biotechnology Division, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 580 089, India,
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  • S. DAYANANDAN,

    1. Forest and Evolutionary Genomics Laboratory, and the Centre for Structural and Functional Genomics, Biology Department, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, Canada H4B 1R6,
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  • N. SREEJAYAN,

    1. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
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  • R. VASUDEVA,

    1. Ashoka Trust for Research in Ecology and the Environment, 5A Main, Hebbal, Bangalore 560 024, India,
    2. Department of Forest Biology, College of Forestry, Sirsi 581 401, India
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  • K. N. GANESHAIAH,

    1. Department of Forestry and Environmental Sciences, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    2. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    3. Ashoka Trust for Research in Ecology and the Environment, 5A Main, Hebbal, Bangalore 560 024, India,
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  • R. UMA SHAANKER

    Corresponding author
    1. Department of Crop Physiology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    2. School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bellary Road, Bangalore 560 065, India,
    3. Ashoka Trust for Research in Ecology and the Environment, 5A Main, Hebbal, Bangalore 560 024, India,
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*Correspondence: R. Uma Shaanker, Fax: (+91)-80-23530070; E-mail: umashaanker@gmail.com

Abstract

Nothapodytes nimmoniana is a medicinally important tree species that occur in the Western Ghats, a megadiversity hotspot in southern India. Inner stem bark of the tree contains an important anti-cancer alkaloid, camptothecin for which the natural population of the tree is heavily harvested. In this paper, we report the isolation and characterization of eight polymorphic microsatellite loci using enrichment hybridization protocol. Analysis of 36 individuals representing two populations revealed three to 12 alleles per locus. Observed heterozygosity ranged from 0.21 to 0.94 for the two populations. None of the loci tested showed linkage disequilibrium. These markers are invaluable for evaluating the genetic structure and assessing the genetic impacts of harvesting of N. nimmoniana in the Western Ghats to formulate strategies for conservation of the species.

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