Isolation and characterization of microsatellites in the harlequin ladybird, Harmonia axyridis (Coleoptera, Coccinellidae), and cross-species amplification within the family Coccinellidae

Authors

  • ANNE LOISEAU,

    1. INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France
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  • THIBAUT MALAUSA,

    1. INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France
    2. INRA, UMR IBSV (INRA/UNSA/CNRS), 400 route des Chappes, BP 167 06903 Sophia-Antipolis Cedex, France
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  • ERIC LOMBAERT,

    1. INRA, UMR IBSV (INRA/UNSA/CNRS), 400 route des Chappes, BP 167 06903 Sophia-Antipolis Cedex, France
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  • JEAN-FRANÇOIS MARTIN,

    1. Montpellier SupAgro, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France
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  • ARNAUD ESTOUP

    1. INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France
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  • doi: 10.1111/j.1755-0998.2009.02517.x

Thibaut Malausa, +33 (0) 4 92386506. E-mail: thibaut.malausa@sophia.inra.fr

Abstract

A total of 18 microsatellite DNA loci were isolated and characterized from the harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae). We optimized a multiplex panel consisting of two polymerase chain reactions, allowing the genotyping of all loci. The number of alleles and heterozygosity observed at each locus ranged from 1 to 12 and from 0 to 100%, respectively. After Bonferroni correction for multiple tests, none of the loci deviated significantly from Hardy–Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. Successful cross-species amplification was obtained for only three of the seven tested species of Coccinellidae.

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