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Identification of the shark species Rhizoprionodon lalandii and R. porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques

Authors

  • F. F. MENDONÇA,

    1. Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista — UNESP, Distrito de Rubião Júnior, s/n, CEP 18618–000, Botucatu, SP, Brazil
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  • D. T. HASHIMOTO,

    1. Laboratório de Genética de Peixes, Departamento de Biologia, Faculdade de Ciências, Universidade Estadual Paulista — UNESP, CEP 17033–360, Bauru, SP, Brazil
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  • F. PORTO-FORESTI,

    1. Laboratório de Genética de Peixes, Departamento de Biologia, Faculdade de Ciências, Universidade Estadual Paulista — UNESP, CEP 17033–360, Bauru, SP, Brazil
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  • C. OLIVEIRA,

    1. Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista — UNESP, Distrito de Rubião Júnior, s/n, CEP 18618–000, Botucatu, SP, Brazil
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  • O. B. F. GADIG,

    1. Campus Experimental do Litoral Paulista, Universidade Estadual Paulista — UNESP, Pça. Infante Dom Henrique, s/n, CEP 11330–900, São Vicente, SP, Brazil
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  • F. FORESTI

    1. Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista — UNESP, Distrito de Rubião Júnior, s/n, CEP 18618–000, Botucatu, SP, Brazil
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Fernando Fernandes Mendonça, Fax: 55-14-3811 6264. E-mail: fernandofm@ibb.unesp.br

Abstract

Rhizoprionodon lalandii and R. porosus are widely distributed along the Atlantic coast of the Americas, living close to coastal areas and therefore frequently captured by seaboard fisheries. However, morphological identification of species in this genus is very difficult, especially when sharks have their heads and fins removed, making information about fishing, trading, and the evaluation of fishery effects on species conservation very difficult. This study's main objective is to develop molecular tools to identify these species using multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism techniques. Both techniques result in good low-cost markers and may be very useful in future studies about the exploitation of these species.

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