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Intron-spanning primers for the amplification of the nuclear ANT gene in decapod crustaceans
Article first published online: 22 JAN 2009
DOI: 10.1111/j.1755-0998.2009.02534.x
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
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How to Cite
TESKE, P. R. and BEHEREGARAY, L. B. (2009), Intron-spanning primers for the amplification of the nuclear ANT gene in decapod crustaceans. Molecular Ecology Resources, 9: 774–776. doi: 10.1111/j.1755-0998.2009.02534.x
Publication History
- Issue published online: 20 APR 2009
- Article first published online: 22 JAN 2009
- Received 5 September 2008; revision accepted 20 November 2008
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Keywords:
- ATP/ADP translocase;
- cryptic species;
- EPIC primers;
- nuclear DNA;
- phylogeography;
- phylogenetics
Abstract
We describe polymerase chain reaction primers that amplify the low-copy nuclear adenine nucleotide transporter gene in decapod crustaceans. These were tested on 35 species from 14 decapod families, and a single polymerase chain reaction product amplified in 32 species. Of 49 sequences generated, only two did not contain an intron, and the longest intron identified was more than 834 nucleotides in length. The amplified fragment is likely to be useful at various taxonomic levels. While the intron is suitable for phylogeographical/population genetic studies and to identify cryptic speciation, the second exon region is sufficiently long to provide signal at both the phylogeographical and phylogenetic levels.