Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae)

Authors

  • MIKE SPIRONELLO,

    1. Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
    Search for more papers by this author
  • LEENA RIZVI,

    1. Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
    Search for more papers by this author
  • DOUGLAS C. CURRIE,

    1. Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
    Search for more papers by this author
  • CHRISTINA M. DAVY,

    1. Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
    Search for more papers by this author
  • ROBERT W. MURPHY

    1. Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario, Canada M5S 2C6, Department of Ecology & Evolution Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
    Search for more papers by this author

  • Deceased (Mike Spironello).

  • doi: 10.1111/j.1755-0998.2009.02544.x

Robert W. Murphy, Fax: 416-586-5553; E-mail: bob.murphy@utoronto.ca

Abstract

Eleven polymorphic microsatellite loci were isolated and developed from the black fly, Simulium negativum, a member of the Simulium arcticum sibling species complex. The observed heterozygosity of the 11 loci ranged from 0.03 to 0.83. The number of alleles per locus ranged from 8 to 19. Significant linkage disequilibria were encountered only for the primer pairs BF7-1 with BF7-5 and BF6-32 with BF7-16. Presumably, these microsatellite loci can be used to study genetic structure within the entire S. arcticum complex.

Ancillary