DNA barcodes for globally threatened marine turtles: a registry approach to documenting biodiversity

Authors

  • EUGENIA NARO-MACIEL,

    1. Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA
    2. Center for Biodiversity and Conservation, American Museum of Natural History, New York, NY 10024, USA
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  • BRENDAN REID,

    1. Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY 10027, USA
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  • NANCY N. FITZSIMMONS,

    1. Institute for Applied Ecology, University of Canberra, Canberra, ACT 2601, Australia
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  • MINH LE,

    1. Center for Natural Resources and Environmental Studies, Vietnam National University, 19 Le Thanh Tong St., Hanoi, Vietnam
    2. Department of Herpetology, American Museum of Natural History, New York, NY 10024, USA
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  • ROB DESALLE,

    1. Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA
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  • GEORGE AMATO

    1. Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA
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Eugenia Naro-Maciel, Fax: +1 212 769 5292; E-mail: enmaciel@amnh.org

Abstract

DNA barcoding is a global initiative that provides a standardized and efficient tool to catalogue and inventory biodiversity, with significant conservation applications. Despite progress across taxonomic realms, globally threatened marine turtles remain underrepresented in this effort. To obtain DNA barcodes of marine turtles, we sequenced a segment of the cytochrome c oxidase subunit I (COI) gene from all seven species in the Atlantic and Pacific Ocean basins (815 bp; n = 80). To further investigate intraspecific variation, we sequenced green turtles (Chelonia mydas) from nine additional Atlantic/Mediterranean nesting areas (n = 164) and from the Eastern Pacific (n = 5). We established character-based DNA barcodes for each species using unique combinations of character states at 76 nucleotide positions. We found that no haplotypes were shared among species and the mean of interspecific variation ranged from 1.68% to 13.0%, and the mean of intraspecific variability was relatively low (0–0.90%). The Eastern Pacific green turtle sequence was identical to an Australian haplotype, suggesting that this marker is not appropriate for identifying these phenotypically distinguishable populations. Analysis of COI revealed a north–south gradient in green turtles of Western Atlantic/Mediterranean nesting areas, supporting a hypothesis of recent dispersal from near equatorial glacial refugia. DNA barcoding of marine turtles is a powerful tool for species identification and wildlife forensics, which also provides complementary data for conservation genetic research.

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