Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers

Authors

  • MARGARET KELLOGG HUNTER,

    1. Sirenia Project, Florida Integrated Science Center, U.S. Geological Survey, 2201 NW 40th Terrace, Gainesville, FL 32605, USA
    2. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Box 100245 UFHSC, Gainesville, FL 32610, USA
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  • DAMIEN BRODERICK,

    1. Molecular Fisheries Laboratory, Queensland Department of Primary Industries and Fisheries, Level 3 Ritchie Building, Research Lane, The University of Queensland, St. Lucia, Qld 4072, Australia
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  • JENNIFER R. OVENDEN,

    1. Molecular Fisheries Laboratory, Queensland Department of Primary Industries and Fisheries, Level 3 Ritchie Building, Research Lane, The University of Queensland, St. Lucia, Qld 4072, Australia
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  • KIMBERLY PAUSE TUCKER,

    1. College of Marine Science, University of South Florida, 140 7th Ave S. MSL 119, St. Petersburg, FL 33701, USA
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  • ROBERT K. BONDE,

    1. Sirenia Project, Florida Integrated Science Center, U.S. Geological Survey, 2201 NW 40th Terrace, Gainesville, FL 32605, USA
    2. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Box 100245 UFHSC, Gainesville, FL 32610, USA
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  • PETER M. MCGUIRE,

    1. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Box 100245 UFHSC, Gainesville, FL 32610, USA
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  • JANET M. LANYON

    1. School of Biological Sciences, The University of Queensland, St. Lucia, Qld 4072, Australia
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Margaret Kellogg Hunter, Fax: (352) 374 8080; E-mail: mkellogg@usgs.gov

Abstract

The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however, robust markers can be difficult and costly to isolate. To increase the number of available markers, dugong and manatee microsatellite primers were evaluated for cross-species amplification. Furthermore, one manatee and four dugong novel primers are reported. After polymerase chain reaction optimization, 23 (92%) manatee primers successfully amplified dugong DNA, of which 11 (48%) were polymorphic. Of the 32 dugong primers tested, 27 (84%) yielded product in the manatee, of which 17 (63%) were polymorphic. Dugong and manatee primers were compared and the most informative markers were selected to create robust and informative marker-panels for each species. These cross-species microsatellite marker-panels can be employed to assess other sirenian populations and can provide beneficial information for the protection and management of these unique mammals.

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