Semi-quantitative differences in gene transcription profiles between sexes of a marine snail by a new variant of cDNA-AFLP analysis

Authors

  • M. MARTÍNEZ-FERNÁNDEZ,

    1. Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Campus As Lagoas-Marcosende Universidad de Vigo, 36310 Vigo, Spain
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  • L. BERNATCHEZ,

    1. Department of Biology, Pavillon Charles-Eugène-Marchand, 1030 Avenue de la Médecine, Université Laval, Québec, QC, Canada G1V 0A6
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  • E. ROLÁN-ALVAREZ,

    1. Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Campus As Lagoas-Marcosende Universidad de Vigo, 36310 Vigo, Spain
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  • H. QUESADA

    1. Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Campus As Lagoas-Marcosende Universidad de Vigo, 36310 Vigo, Spain
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E. Rolán-Alvarez, Fax: +34 986 813828; E-mail: rolan@uvigo.es

Abstract

A variant of the cDNA-AFLP method coupled to an automated sequencer was used to quantify transcripts differentially expressed between sexes of the marine snail Littorina saxatilis. First, we conducted a validation study of the technique using known concentrations of a commercial marker. Second, we analysed six replicates of males and females from a population showing no apparent sexual dimorphism. The results confirm that the method can be properly used within the range of DNA concentrations utilized. In addition, we detected a small percentage of spots (1.8%) differentially expressed between sexes, as expected from a low to moderately sexual dimorphic species.

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