Get access

New chloroplast primers for intraspecific variation in Dicranum scoparium Hedw. (Dicranaceae) and amplification success in other bryophyte species

Authors

  • A. LANG,

    1. Unité de Phylogénie et de Génétique Moléculaires, Conservatoire et Jardin Botaniques. Chemin de l’Impératrice, 1. Case Postale 60. 1292 Chambésy, Geneva, Switzerland
    Search for more papers by this author
  • Y. NACIRI

    1. Unité de Phylogénie et de Génétique Moléculaires, Conservatoire et Jardin Botaniques. Chemin de l’Impératrice, 1. Case Postale 60. 1292 Chambésy, Geneva, Switzerland
    2. Laboratoire de Systématique Végétale et Biodiversité, Université de Genève, Chemin de l’Impératrice, 1. Case Postale 60. 1292 Chambésy, Geneva, Switzerland
    Search for more papers by this author

Y. Naciri, Fax: +41 22 418 5101; E-mail: yamama.naciri@ville-ge.ch

Abstract

Primers for four loci that amplify cpDNA regions have been designed for population genetic analyses in Dicranum scoparium Hedw. and compared with trnL(UAA)5′exon-trnF. All loci showed intraspecific variation with a number of haplotypes ranging between two and six. trnH-psbADic showed an intercontinental disjunction, but no variability within the four Swiss populations surveyed, whereas the three remaining loci displayed intrapopulation variability in at least one population (rps19-rpl2, rpoB, trnT-rps4). These primers were additionally tested on 22 bryophytes and three fern species. The primers amplified mostly in mosses and liverworts, but less well in ferns, pointing to their evolutionary distance from the bryophytes.

Ancillary