Both authors contributed equally.
Multiplex assay to identify Korean vectors of malaria
Article first published online: 19 FEB 2010
© 2010 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 10, Issue 4, pages 748–750, July 2010
How to Cite
JOSHI, D., PARK, M. H., SAEUNG, A., CHOOCHOTE, W. and MIN, G. S. (2010), Multiplex assay to identify Korean vectors of malaria. Molecular Ecology Resources, 10: 748–750. doi: 10.1111/j.1755-0998.2010.02835.x
- Issue published online: 11 JUN 2010
- Article first published online: 19 FEB 2010
- Received 5 November 2009; revision received 5 January 2010; accepted 8 January 2010
Fig. S1 Comparision done between this study and the assay previously described by Li et al. (2005). M: 1-kb DNA marker (Enzymonics, Korea). Lanes 1–8 were identified by multiplex primers developed in this study: lanes 1–4, Anopheles kleini (385 bp); and lanes 5–8, Anopheles belenrae (485 bp). Lanes 9–16 were assayed by primers of Li et al. (2005): lanes 9–12, An. kleini (288 bp); and lanes 13–16, An. belenrae (287 and 162 bp).
Fig. S2 Aligned ribosomal DNA (rDNA) sequences from six indigenous species of Anopheles Hyrcanus group from South Korea. The extended sequences covered partial 5.8S, whole internal transcribed spacer 2 (ITS2) and extended 28S regions. ITS2 forward = 5.8S-based universal forward primer, ANO28S+660 = 28S-based internal sequencing primer and ANO 28S-R = 28S-based universal reverse primer. Arrows indicate the species-specific primer loci. Arrows also demarcate the boundaries of the ITS2, D1, D2 and D3 regions of rDNA.
Table S1 Randomly identified wild-caught mosquitoes from different parts of Korea (Sore, Paju and Youncheon) and selected number of sequencing from batch specimens
Table S2 Inter-specific sequence variations within the rDNA regions of six Korean members of the Hyrcanus group. Lower-left and upper-right are variations (%) within internal transcribed spacer 2 and extended 28S regions
Table S3 Inter-specific sequence variations among six indigenous members of the Korean Anopheles Hyrcanus group. Lower-left and upper-right are variations (%) within D1 and D2 region of 28S rDNA
Table S4 Inter-specific sequence variation among six indigenous members of the Korean AnophelesHyrcanus group within the D3 region of 28SrDNA
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