Fig. S1 Map showing sampling localities of red-legged (black square) and barbary (white square) partridges (Table S1). The position of the Experimental Farm of the Italian Forest Service of Bieri is indicated as well (white triangular, in central Italy). Alectoris barbara × A. rufa hybrids were produced in Bieri, Tuscany (2003–2008).

Table S1 Specimens used in this study. Locality, country, population type, and sample tissue type are reported for A. barbara controls (n = 30), A. rufa controls (n = 80) and experimental A. barbara × A. rufa hybrids (n = 125)

Table S2 Consensus sequence identity matrix for A. rufa (upper part, Ar) and A. barbara (lower part, Ab): OP-A-05 and OP-AZ-01 data are reported above and below the diagonal, respectively. Nucleotide identity values are expressed as percentage and computed using the consensus sequences obtained for each parental and hybrid (F1, F2, F3, BC1, BC2) category

Table S3 Individual RAPD score is reported for each specimen within all investigated generations (F1, F2, F3, BC1, BC2) of known A. barbara × A. rufa hybrids. Presence/absence of species-specific bands is reported as 1/0. Abbreviations: Ar, A. rufa; Ab, A. barbara

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