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A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex

Authors

  • I. KELLER,

    1. Department of Fish Ecology and Evolution, EAWAG Swiss Federal Institute of Aquatic Science and Technology, Center of Ecology, Evolution and Biochemistry, Seestrasse 79, CH-6047 Kastanienbaum, Switzerland
    2. Department of Aquatic Ecology, EAWAG Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, CH-8600 Dübendorf, Switzerland
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  • A. TAVERNA,

    1. Department of Fish Ecology and Evolution, EAWAG Swiss Federal Institute of Aquatic Science and Technology, Center of Ecology, Evolution and Biochemistry, Seestrasse 79, CH-6047 Kastanienbaum, Switzerland
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  • O. SEEHAUSEN

    1. Department of Fish Ecology and Evolution, EAWAG Swiss Federal Institute of Aquatic Science and Technology, Center of Ecology, Evolution and Biochemistry, Seestrasse 79, CH-6047 Kastanienbaum, Switzerland
    2. Department of Aquatic Ecology and Macroevolution, Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland
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Irene Keller, Fax: +41 44 823 5028; E-mails: irene.keller@eawag.ch

Abstract

The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout. Pyrosequencing-based assays for molecular species identification are expected to be generally useful whenever multiple positions in a short DNA sequence need to be assessed.

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