A suite of twelve single nucleotide polymorphism markers for detecting introgression between cutthroat and rainbow trout

Authors

  • ANDREW S. HARWOOD,

    1. Salmonid Genetics Laboratory, School of Biological Sciences, Washington State University Vancouver, 14204 NE Salmon Creek Ave, Vancouver, Washington 98686, USA
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  • RUTH B. PHILLIPS

    1. Salmonid Genetics Laboratory, School of Biological Sciences, Washington State University Vancouver, 14204 NE Salmon Creek Ave, Vancouver, Washington 98686, USA
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Andrew S. Harwood, Fax: (360) 546 9064; E-mail: andrew.harwood@email.wsu.edu or andrewharwood36@hotmail.com

Abstract

A suite of 12 subspecies and species-specific single nucleotide polymorphism (species-specific SNP) markers was developed to distinguish rainbow trout (RT) Oncorhynchus mykiss from the four major subspecies of cutthroat trout: westslope cutthroat trout (WCT) Oncorhynchus clarki lewisi, Yellowstone cutthroat trout (YCT) Oncorhynchus clarki bouvieri, coastal cutthroat trout (CCT) Oncorhynchus clarki clarki, Lahontan cutthroat trout (LCT) Oncorhynchus clarki henshawi, and their hybrids. Several of the markers were linked to help strengthen hybrid determinations, and sex-specific species-specific SNP assays were also developed.

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