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A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus

Authors

  • C. MURATA,

    1. Graduate School of Life Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
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  • G. OGURA,

    1. Laboratory of Animal Functions Science, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan
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  • A. KUROIWA

    1. Graduate School of Life Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
    2. Laboratory of Animal Cytogenetics, Faculty of Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
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Asato Kuroiwa, Fax: +81 11 736 6304; E-mail: asatok@cris.hokudai.ac.jp

Abstract

To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769-bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546-bp fragment amplified only from male DNA samples was derived from EIF2S3Y. The results indicated that this primer set is useful for sex identification in this species.

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