Present address: Department of Systematics, Evolution and Climate Change, Biodiversity and Climate Research Centre (BiK-F) & Goethe University, Senckenberganlage 25, D-60325 Frankfurt, Germany.
1755-0998/asset/olbannercenter.gif?v=1&s=461bba13dc862037c63fd362e625b54da4c20f13)
DNA BARCODING
Evaluation of candidate DNA barcoding loci for economically important timber species of the mahogany family (Meliaceae)
Article first published online: 6 FEB 2011
DOI: 10.1111/j.1755-0998.2011.02984.x
© 2011 Blackwell Publishing Ltd
Additional Information
How to Cite
MUELLNER, A.N., SCHAEFER, H. and LAHAYE, R. (2011), Evaluation of candidate DNA barcoding loci for economically important timber species of the mahogany family (Meliaceae). Molecular Ecology Resources, 11: 450–460. doi: 10.1111/j.1755-0998.2011.02984.x
- †
Present address: Department of Systematics, Evolution and Climate Change, Biodiversity and Climate Research Centre (BiK-F) & Goethe University, Senckenberganlage 25, D-60325 Frankfurt, Germany.
- ‡
Present address: Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Publication History
- Issue published online: 12 APR 2011
- Article first published online: 6 FEB 2011
- Received 1 October 2010; revision received 3 December 2010; accepted 14 December 2010
Keywords:
- Cedrela;
- DNA barcoding;
- mahogany;
- Meliaceae;
- Sapindales;
- Swietenia
Abstract
There has been considerable debate regarding locus choice for DNA barcoding land plants. This is partly attributable to a shortage of comparable data from proposed candidate loci on a common set of samples. In this study, we evaluated main candidate plastid regions (rpoC1, rpoB, accD) and additional plastid markers (psbB, psbN, psbT exons and the trnS-trnG spacer) as well as the nuclear ribosomal spacer region (ITS1-5.8S-ITS2) in a group of land plants belonging to the mahogany family, Meliaceae. Across these samples, only ITS showed high levels of resolvability. Interspecific sharing of sequences from individual plastid loci was common. The combination of multiple loci did not improve performance. DNA barcoding with ITS alone revealed cryptic species and proved useful in identifying species listed in Convention on International Trade of Endangered Species appendixes.