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Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea)

Authors

  • GLENN YANNIC,

    1. Department of Ecology and Evolution, University of Lausanne, CH-1015 Lausanne, Switzerland
    2. Groupe de Recherche en Ecologie Arctique, 16 rue de Vernot, F–21440 Francheville, France
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    • Present address: Département de biologie and Centre d’études nordiques, Université Laval, 1045 avenue de la Médecine, Québec G1V 0A6, Canada

  • ROBERTO SERMIER,

    1. Department of Ecology and Evolution, University of Lausanne, CH-1015 Lausanne, Switzerland
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  • ADRIAN AEBISCHER,

    1. Groupe de Recherche en Ecologie Arctique, 16 rue de Vernot, F–21440 Francheville, France
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  • MARIA V. GAVRILO,

    1. Arctic and Antarctic Research Institute (AARI), 38 Bering Street, Saint-Petersburg, 199397, Russia
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  • OLIVIER GILG,

    1. Groupe de Recherche en Ecologie Arctique, 16 rue de Vernot, F–21440 Francheville, France
    2. Université de Bourgogne, Laboratoire Biogéosciences, UMR CNRS 5561, Equipe Ecologie Evolutive, 6 Boulevard Gabriel, 21000 Dijon, France
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  • CECILIE MILJETEIG,

    1. Norwegian Polar Institute, Polar Environmental Centre, 9296 Tromsø, Norway
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  • BRIGITTE SABARD,

    1. Groupe de Recherche en Ecologie Arctique, 16 rue de Vernot, F–21440 Francheville, France
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  • HALLVARD STRØM,

    1. Norwegian Polar Institute, Polar Environmental Centre, 9296 Tromsø, Norway
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  • EMMANUELLE POUIVÉ,

    1. ECV, rue du Valentin, 7, 1004 Lausanne, Switzerland
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  • T. BROQUET

    1. Department of Ecology and Evolution, University of Lausanne, CH-1015 Lausanne, Switzerland
    2. CNRS, UMR 7144, Team Div&Co, Lab. Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29682 Roscoff, France
    3. UPMC Univ Paris 06, UMR 7144 AD2M, Station Biologique de Roscoff, 29682 Roscoff, France
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Glenn Yannic, Fax: 1-418-656-2043; E-mail: Glenn.Yannic@gmail.com

Abstract

We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n = 21) and Russia (Severnaya Zemlya Archipelago, n = 21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy–Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull.

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