Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development
Article first published online: 16 JUN 2011
© 2011 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 11, Issue 6, pages 1093–1101, November 2011
How to Cite
GARDNER, M. G., FITCH, A. J., BERTOZZI, T. and LOWE, A. J. (2011), Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development. Molecular Ecology Resources, 11: 1093–1101. doi: 10.1111/j.1755-0998.2011.03037.x
- Issue published online: 13 OCT 2011
- Article first published online: 16 JUN 2011
- Received 5 April 2011; revision received 2 May 2011; accepted 13 May 2011
Appendix S1 Characteristics of the 454 shotgun sequencing runs and the results of microsatellite development in the 54 species used in the study.
Fig. S1 Mean standardized number of sequences (see text) obtained from the 454 runs within broad taxonomic groupings. Error bars represent ±1 SE.
Fig. S2 The percentage of each motif class identified in the 454 runs. See text for search parameters and membership of each broad taxonomic group.
Fig. S3 Regression of arcsine transformed % GC and average fragment length of 454 sequences.
Fig. S4 The average percent of a 454 plate required to obtain 40 unique microsatellite loci with primers designed for each of the broad taxonomic groups. Error bars represent ±1 SE.
|MEN_3037_sm_AppendixS1.xls||55K||Supporting info item|
|MEN_3037_sm_SuppFiguresS1-S4.doc||131K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.