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Appendix S1 Characteristics of the 454 shotgun sequencing runs and the results of microsatellite development in the 54 species used in the study.

Fig. S1 Mean standardized number of sequences (see text) obtained from the 454 runs within broad taxonomic groupings. Error bars represent ±1 SE.

Fig. S2 The percentage of each motif class identified in the 454 runs. See text for search parameters and membership of each broad taxonomic group.

Fig. S3 Regression of arcsine transformed % GC and average fragment length of 454 sequences.

Fig. S4 The average percent of a 454 plate required to obtain 40 unique microsatellite loci with primers designed for each of the broad taxonomic groups. Error bars represent ±1 SE.

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