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Table S1 Samples and voucher information for the Holcoglossum species used in this study.

Table S2 PCR and sequencing primers used in this study.

Table S3 Character-based DNA database for Holcoglossum species from the matK region.

Table S4 Character-based DNA database for Holcoglossum species from the ITS region.

Table S5 Character-based DNA database for Holcoglossum species from the trnH-psbA region.

Fig. S1 Bayesian inference tree (BI, left) and neighbour joining tree (NJ, right) based on the matK matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. White circles indicate that a single diagnostic site was used; black ones mean that a combination of candidate sites was used; stars and asterisks represent those species that had the same sequences. The species in bold was identified only through the NJ tree.

Fig. S2 Bayesian inference tree (BI, left) and neighbour joining tree (NJ, right) based on the ITS matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. White circles indicate that a single diagnostic site was used; black ones mean that a combination of candidate sites was used; stars and asterisks represent those species that had the same sequences. The species in bold was identified only on the NJ tree.

Fig. S3 Bayesian inference tree (BI) based on the trnH-psbA matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. White circles indicate that a single diagnostic site was used; black ones mean that a combination of candidate sites was used; stars and asterisks represent those species that had the same sequences.

Fig. S4 Bayesian inference tree (BI, left) and neighbour joining tree (NJ, right) based on the matK+trnH-psbA combined matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. The species in bold could be identified on the basis of loci examination.

Fig. S5 Bayesian inference tree (BI) based on the ITS+trnH-psbA combined matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. The species in bold could be identified on the basis of loci examination.

Fig. S6 Bayesian inference tree (BI) based on the matK+trnH-psbA+ITS combined matrix for 12 Holcoglossum species. Numbers on branches represent BI and NJ support values, respectively. Every individual is shown with its DNA number. The species in bold could be identified on the basis of loci examination.

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